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KMID : 0379119900180010007
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Korean Journal of Mycology
1990 Volume.18 No. 1 p.7 ~ p.12
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Production of Pectolytic Enzymes by Penicillium expansum
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Abstract
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Isolates of Penicillium expansum with reduced pathogenicity were arbitrarily selected among benomyl-resistant isolates in order to investigate relationship of their pectolytic enzyme acitivity with pathogenicity. In artificial medium, strongly pathogenic isolate S₁ and weakly pathogenic isolate R₂ produced considerable amonts of endo-polymethylgalacturonase, endo-polygalacturonase, pectin methyl-trans-eliminase, and polygalacturonate-trans-eliminase. No marked difference in enzyme activities was observed between two isolates. In apple medium, the activities of endo-polymethylgalacturonase and endo-polygalacturonase of isolate S₁ were over 6 times higher than those of isolate R₂. But pectin methyl-trans-eliminase and polygalacturonate-trans-eliminase did not show a great difference. Activities of endo-polymethylgalacturonase and endo-polygalacturonase precipitated at 80-95% saturation of ammonium sulfate were highest, and addition of these enzyme solutions increased pathogenicity of weakly pathogenic isolates R_(1-4).
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